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PRIMARY PRODUCTIVITY CHANGES


A description of the method was written February 2010 by D. Wolgast.  Changes to the method are listed below.


Radioactive Specific Activity Changes

Section Date Prepared By Description
       
3.3 6/10/2016 D. Wolgast 25mCi diluted to 32uCi/ml Batch 0509YW4, 800mls
3.3 8/8/2014 D. Wolgast New 14C Stock: diluted to 69.40µCi/ml; Batch 0500ZV4 (replaces 0509G57 orig. UCSD barcode), used for (1411) November 2014 to present
3.3 9/8/2010 D. Wolgast & D. Faber New 14C Stock: diluted to 308.37µCi/ml; Source: MP Biomedicals, barcode 0508YD5, used for (1011) September 2010 until July 2014 (1407)
3.3 12/15/2008 D. Wolgast & D. Faber New 14C Stock: diluted to 284.23 µCi/ml; Source: MP Biomedicals, batch 081215, used for (0901) January 2009 until August 2010 (1008)
3.3 3/9/2007 D. Wolgast & J. Sheldon New 14C Stock: diluted to 271.32 µCi/ml; Source: MDS Nordion, batch 070308, used for (0704) April 2007 until November 2008 (0810)
3.3 6/23/2005 D. Wolgast New 14C Stock: diluted to 335.90 µCi/ml; Source: MP Biomedicals, batch 0506, used for (0507) July 2005 until January 2007 (0701)
3.3 4/5/2005 D. Wolgast New 14C Stock: diluted to 215.76 µCi/ml; Source: MP Biomedicals, used for (0504) April 2005
3.3 10/25/2004 D. Wolgast New 14C Stock: diluted to 2351.46µCi/ml; Batch 0411, used for (0411) November 2004 until January 2005 (0501)
3.3 3/3/2004 J. Sheldon New 14C Stock: diluted to 75.03 µCi/ml; Source: ICN Biomedicals, batch 0402, used for (0403) March 2004 until July 2004 (0407)
3.3 Mar-03 D. Wolgast New 14C Stock: diluted to 41.48 µCi/ml; Source: ICN Biomedicals, batch 0324, used for (0304) April 2003 until January 2004 (0401)
3.3 Mar-03 D. Wolgast New 14C Stock: diluted to 42.38 µCi/ml; Source: ICN Biomedicals, batch 0302, used for (0204) April 2002 until February 2003 (0302)
3.3 11/17/1995 D. Wolgast New 14C Stock: diluted to 51.45 µCi/ml; Source: ICN Biomedicals,batch 03Z58 used for Nov 1995 until  January 2002 (0201)

Radioactivity added

Cruise Radioactivity added µCi Comments
1704    
1701 5.89  
1611 6.58  
1607 6.63 New Batch 0509YW4
1604 9.26  
1601 8.64  
1511 10.36  
1507 11.64  
1504 12.18  
1501 8.74; 8.08 Due to the decreasing activity of the isotope stock through the cruise the prodo samples were processed in two batches.
1411 10.21  
1407 7.783  
1404 9.481  
1402 9.47  
1311 10.239  
1307 11.47  
1304 11.03  
1301 11.28  
1210 Varied  
1207 27.68; 12.95  
1203 12.69  
1202 12.36  
1110 11.76; 10.15 Due to the decreasing activity of the isotope stock through the cruise the prodo samples were processed in two batches.
1108 13.78  
1104 6.992  
1101 49.66  
1011 50.64  
1008 25.6  
1004 40.05  
1001 40.6  
0911 43.19  
0907 43.38  
0903 45.75  
0901 46.59  
0810 40.27  
0808 44.36  
0804 45.8  
0801 52.29  
0711 47.95  
0707 48.55  
0704 53.14  
0701 44  
0610 56.1  
0607 63.1  
0604 59.1  
0602 59.1  
0511 59.1  
0507 64.74  
0504 41.58  
0501   Variable due to problems with acid/Teflon and bicarbonate substrate.
0411 32.3-41.66 Transition to higher activity to facilitate DO14C assay.
0407 15  
0404 15  
0401 8.3  
0310 8.3  
0307 8.3  
0304 8.3  
0302 8.48  
0211 8.48  
0207 8.48  
0204 8.48 Stock diluted and stored in cleaned polycarbonate and Teflon
0201 11.3 Stock in glass sealed ampoules
0110 11.3 Stock in glass sealed ampoules
0107 11.3 Stock in glass sealed ampoules
0104 11.3 Stock in glass sealed ampoules
0101 11.3 Stock in glass sealed ampoules
0010 11.3 Stock in glass sealed ampoules
0007 11.3 Stock in glass sealed ampoules
0004 11.3 Stock in glass sealed ampoules
0001 11.3 Stock in glass sealed ampoules
9910 11.3 Stock in glass sealed ampoules
9908 11.3 Stock in glass sealed ampoules
9904 11.3 Stock in glass sealed ampoules
9901 11.3 Stock in glass sealed ampoules

Methodological Changes

Section

Date

Author

Description

       

 

Apr-05

D. Wolgast

The procedure for calculating cruise 14C specific activity for the productivity assay was changed to reflect daily 14C additions averaged over the course of a cruise.  the six dark bottles have one milliliter removed, added to ethanolamine spiked scintillation cocktail for counting.  Previously specific activity was calculated for a batch of stock and used for the entire cruise.  The new method served to check pipeting, inoculation amounts and any changes in volatile 14C stocks.

0411

 

D. Wolgast

Transition to higher activity to facilitate DO14 C assay, 10ml split removed from samples for 14 C

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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