National Oceanic and Atmospheric Administration

NATIONAL MARINE FISHERIES SERVICE SOUTHWEST FISHERIES SCIENCE CENTER 8604 LA JOLLA SHORES DRIVE LA JOLLA, CA 92037

February 18, 2005 F/SWC1:DAG

PRELIMINARY CRUISE ANNOUNCEMENT

VESSEL:NOAA Vessel David Starr Jordan, 0504-JD, DS 05-03.

CRUISE DATES: March 27 - April 25, 2005.

PROJECT: Pelagic Biomass/CalCOFI, Fisheries Resources Division.

ITINERARY: Leg I: Depart San Diego, California at 1000 on March 27, 2005. Proceed to first station 95.0/28.0 (position 32°37.1'N/117° 12.2'W) and begin the proposed pattern (see attached cruise track). To complete the proposed cruisetrack, it will be necessary to maintain maximum speed betweenstations whenever possible. On or around April 4, the shipwill exchange scientific personnel in Avila Beach, California.Once station 73.3/100 is reached, standard CalCOFI operationswill begin on lines 73.3, 70.0, 66.7, 63.3 and 60.0. Aftercompleting line 66.7, the ship will pull into San Francisco tofuel on or around April 12, 2005.

Leg II: The ship will depart San Francisco after fueling andresume operations on line 65.0 until the proposed pattern iscompleted. If time allows, trawling operations will continueduring the transit back to San Diego. The vessel will returnto San Diego, California on April 25, 2005.

OBJECTIVES: 1.The National Marine Fisheries Service (NMFS) and the California Department of Fish and Game (CDFG) have theresponsibility of determining the status of the Pacificsardine (Sardinops sagax) population along the west coast ofNorth America. During the 2005 sardine survey, data will becollected to determine the biomass of the sardine populationlocated between San Francisco as the northern boundary and SanDiego as the southern boundary. These data will be analyzedusing a daily egg production method (DEPM) to determine thespawning biomass. This method measures the abundance of newlyspawned eggs and the rate at which mature females are producing eggs.

To conduct continuous underway sampling of surface waters.Temperature and salinity will be automatically logged bycomputer with the output from the GPS navigational unit.
To record current profiles throughout the duration of thecruise with the Acoustic Doppler Current Profiler in an attempt to estimate net transport in the northern region.
To continue an ongoing assessment of pelagic fish stocksbetween La Jolla and San Francisco, California.
To monitor environmental conditions within the CalCOFI survey area.
To make continuous observations of sea birds and marine mammals.
To record continuous acoustic targets obtained with the EK-60scientific sounder.
Collect fishery independent adult sardines for spawningbiomass estimates.

PROCEDURES: 1.The Jordan will conduct operations in conjunction with theScripps Institution of Oceanography research vessel New Horizon. During the southern occupation of the pattern(roughly, San Diego to Point Conception), the Jordan will occupy intermediate lines of the CalCOFI pattern while the New Horizon conducts standard CalCOFI operations on the cardinallines (see attached diagram). During this survey, the Jordan will conduct directed adaptive sampling of pelagic fish eggsusing the following protocol: Water will be continuouslysampled using the CUFES (Continuous Underway Fish Egg Sampler)from a depth of three meters. Approximately 640 liters/minuteis sent through a concentrator which filters all materiallarger than 505 µm. The sieved material is then collected and identified. All fish eggs are identified to lowest taxa,counted and entered into the data acquisition software.

Sampling intervals will vary in length, depending on thenumber of fish eggs seen, from five to 30 minutes. If two consecutive samples have a concentration of Pacific sardineeggs equal to or greater than 1 egg per minute, the ship willstop to conduct a Pairovet tow. Pairovet tows will continue at four mile intervals until a concentration of less than one egg per minute is observed in two consecutive samples. Thus,the offshore extent of each line will be determined by theabsence of sardine eggs. All Pairovet samples will be takenconcurrently with CUFES samples in addition to samplingcontinuously between Pairovet samples. The four mile interval may be adjusted if SST’s from satellite images suggests apotentially large spawning habitat.

The Pairovet net will be fished from 70 meters to the surface (depth permitting) using paired 25 cm diameter 150 µm meshnets. The technical requirements for Pairovet tows are:Descent rate of 70 meters per minute, a terminal depth time of10 seconds and an ascent rate of 70 meters per minute. All tows with wire angles exceeding 15° during the ascent will berepeated.

Once the Jordan has completed the southern survey, up to line75.0, full CalCOFI stations will be occupied starting on line
73.3 and will continue on lines 70.0, 66.7, 63.3 and 60.0 outto station 100. The intermediate lines in the northern regionwill be surveyed using an adaptive approach similar to thesouthern region.

Each standard CalCOFI station will include the following:

A CTD/Rosette consisting of 12 2-liter hydrographicbottles will be lowered to 500 meters (depth permitting)to measure physical parameters and collect water atdepth, mixed layer and surface for calibration analysisof oxygen, salinity, and chlorophyll concentrations.
A CalBOBL (CalCOFI Bongo) standard oblique plankton towwith 300 meters of wire out, depth permitting, usingpaired 505 µm mesh nets with 71 cm diameter openings.

The technical requirements for this tow are: Descentrate of 50 meters per minute. All tows with ascendingwire angles lower than 38° or higher than 51° in thefinal 100 meters of wire will be repeated.Additionally, a 45° wire angle should be closelymaintained during the ascent and descent of the netframe.

c.: A Manta net (neuston) tow, using a 505 µm mesh net on aframe with a mouth area of 0.1333 m².
d.: Weather observations.
e.: A Pairovet (vertical) plankton tow will be taken usingprotocols as described previously.
f.: During transit between stations, a bird observer will berecording location and species of various sea birds andmarine mammals.

5. A thirty minute surface tow using SWFSC’s Nordic 264 surfacetrawl with 3.0 m² foam core doors will be performed duringevening hours. An attempt will be made to conduct as manytrawls as possible when the ship is in areas of possible adultsardine occurrence. The trawl will be fitted with Polyformfloats at the trawls wings and headrope.

EQUIPMENT: 1. Supplied by scientific party:

! -80°C Freezer (SWFSC)
! 37% Formalin (SWFSC)
! Ethanol (SWFSC)
! Tris buffer (SWFSC)
! Sodium borate (SWFSC)
! 30 cc and 50 cc syringes (SWFSC)
! Canulas (SWFSC)
! Pint, quart and gallon jars (SWFSC)
! Scintillation vials (SWFSC)
! Inside and outside labels (SWFSC)
! CalCOFI net tow data sheets (SWFSC)
! 71 cm CalCOFI Bongo frames (SWFSC)
! 71 cm CalCOFI 505 µm mesh nets (SWFSC)
! CalCOFI 150 µm Calvet nets and codends (SWFSC)
! CalCOFI Pairovet frames (SWFSC)
! 333 µm mesh codends (SWFSC)
! Inclinometer for bongo tows (SWFSC)
! Digital flowmeters (SWFSC)
! 75 lb Bongo weight (SWFSC)
! 100 lb hydro weights (SWFSC)
! CalCOFI Manta net frames (SWFSC)
! 60 cm CalCOFI 505 µm mesh Manta nets (SWFSC)
! Standard CalCOFI tool boxes (SWFSC)
! Bucket thermometers and holders (SWFSC)
! Hand held inclinometer (SWFSC)
! Guildline Portasal (SWFSC)
! Salinity bottles (SWFSC)
! Standard sea water (SWFSC)
! Data sheets for scheduled hydrographic work (SIO)
! Weather observation sheets (SIO)
! CTD and rosette (SWFSC)
! 2 liter hydrographic bottles (SWFSC)
! EK-60 Simrad Scientific sounder (SWFSC)
! CUFES (SWFSC)
! CUFES data acquisition software (SWFSC)

! CUFES data acquisition notebook computer (SWFSC)! 2 Nordic 264 midwater trawls (SWFSC)! 3.0 m² foam core trawl doors (SWFSC)! Trawl rigging (SWFSC)

2. Supplied by David Starr Jordan:

! Starboard hydro winch with ¼” cable for standard Bongo,

Pairovet and Manta tows ! Port winch with .322" conductive cable ! Constant temperature room set at 22°C ±1°C (71.5°F ±2°F)! Winch monitoring system! Seabird thermosalinometer ! Knudsen bottom sounder ! Acoustic Doppler Current Profiler w/writeable CD drive! Serial cable from SCS for egg pump data acquisition

computer located in port specimen lab

!Combo winch with e" cable, port and starboard drum

MISCELLANEOUS:

The disposal of fish caught will be in accordance with NOAAAdministrative order 202-735B dated January 25, 1989.
At the completion of the cruise an inspection will be made ofscientific working and berthing spaces by the CommandingOfficer or his designated representative. The Scientific party is responsible for the condition and cleanliness ofspaces assigned to the scientific party.
The Cruise Leader will hold a pre-cruise meeting aboard thevessel before departure.
The Cruise Leader will hold a post-cruise meeting upontermination of the cruise.
NOAA Fleet Medical Policy requires that all scientific personnel embarking on NOAA vessels complete a NOAA HealthServices Questionnaire and have it approved through OMAO.
All dates and times recorded will be in Pacific Standard Time.

PERSONNEL: Leg I:Dave Griffith, Cruise Leader SWFSC Bev Macewicz SWFSC Ron Dotson SWFSC TBD SWFSC TBD SWFSC Marguerite Blum* MBARI TBD* MBARI Bird observer PRBO

Leg II:Dave Griffith, Cruise Leader SWFSC Bev Macewicz SWFSC Ron Dotson SWFSC TBD SWFSC Marguerite Blum MBARI TBD MBARI Bird observer PRBO

* Will board in Avila Beach SWFSC personnel authorized per diem at the rate of $3.00 per day tobe paid via the Imprest Fund at the termination of the cruise.

WATCH HOURS: 1800-0559 Charge to account C8LAF40-P150600-1759

Date: Prepared by: D.A. Griffith

Approved by:William W. Fox, Ph.DScience & Research Director Southwest Region

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