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CTD
– stands for Conductivity-Temperature-Depth
and refers to the electronic instrument deployed into the ocean to
measure these and other parameters. Electronic sensors measure
different aspects of the ocean while the CTD is lowered to a terminal
depth (on CalCOFI ~500m, depth permitting). These data are
transmitted up the conductive cable and display on the CTD computer
screen real-time. The screen plots created during the
downcast are used to determine the upcast optimal bottle closure
depths/spacing. After being lowered to the terminal depth
without stopping, the rosette (array of 24 bottles
mounted on the frame that surrounds the CTD electronics) bottles are
sequentially closed at specific target depths as the CTD is
raised. Although the terms CTD or rosette may be used
interchangeably when referring to the complete package, the CTD is the
electronic portion; the rosette and carousel
(central hub that controls the bottle closure) are the bottle
array. The CTD electronics may be deployed independent of the
rosette.
rosette
– metal frame and bottle array that
surrounds the CTD electronics. CalCOFI uses 24 ten liter
bottles; other programs may use 6, 12, 24, or 36 bottles of various
volumes. The carousel (or pylon)
holds the bottle lanyards until the CTD data
aquisition programs sends a command to close a specific
bottle. CalCOFI’s bottle closure sequence is bottle
#1 at terminal depth (usually ~500m) ending with bottle #20 at surface
(~2m). The 4 extra bottles are for discretionary use (except
the Santa BarbaraBasin station where all 24 are closed).
Carousel (or pylon)
– electronic hub centered on the rosette that holds the
bottles open until it receives the release command. Bottle
closure is usually sequential but the software can be program to close
the bottle in any order.
Lanyard – nautical term
used to describe a short line or rope that secures or rigs
something. In reference to the CTD/rosette, the nylon rigging
used to keep the bottle top & bottom caps open
HPLC- High-pressure liquid
chromatography; high-performance liquid chromatography.
Fluorometer -instrument used to
measure fluorescent materials; for Chl-a, it emits a excitation
wavelength of light ~460nm and detects the emission return at
~685nm.
Fluorescence - the phenomena of some
compounds to absorb specific wavelengths of light and instantaneously
emit longer wavelengths of light energy
Chlorophyll a -
the green photosynthetic pigment contained in all living algae that can
be directly measured and used as the primary indicator of algal biomass
Chlorophyll b -
an accessory pigment found in some algae that can effect the accuracy
of chlorophyll a determinations
Extraction - the process of using a
solvent (acetone) to remove the chlorophyll from the algal cells.
Eutrophic - term used to describe a
nutrient-rich body of water.
In vivo - Term used for measuring
chlorophyll that is contained naturally within the algal cells
In-Situ - Term synonymous with in
vivo
In-vitro - Term used when measuring
chlorophyll that is extracted from the algal cells
Oligotrophic - term used to describe
a nutrient-poor body of water.
Pheophytin - term for degraded
chlorophyll that occurs naturally or as a result of acidification.
Phytoplankton - the photosynthesizing
constituent of plankton, mainly unicellular algae
Phycoerythrin - the accessory pigment
found primarily in marine cyanobacteria species such as
Synechococcusspp
Primary Standard - a sample of the
exact same material being measured, and ideally at a known
concentration level.
Quenching - term refers to factors
that reduce, or quench, fluorescence.
Secondary Standard - a sample
material that can be used as a secondary measure for calibration, and
provides a stable reference that is repeatable.
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