- CTD prep – draining and rigging of CTD for deployment 20mins before station. Rosette bottles are drained, bottom lanyards unclipped; once drained, all valves are closed and rotated 90°; breathers are closed (lightly finger-tight).
- CTD launching and recovery - help untie the deck lines; handle tag lines to keep the package from swinging during deployment; hook the CTD on recovery and help land the CTD safely on deck; tie the CTD back down
- Sample Drawing –
- Refer to the sample log sheet for the bottle numbers to sample. The number of samples and the bottle they start on can vary - shallow stations have fewer bottles; others may have extra bottles with additional samples to draw. Certain sample types may not be drawn from all the bottles.
- Standard station samples drawn are:
- Oxygens – must be drawn first to minimize contamination, usually by the CTD operator
- Salts – very sensitive to fresh water contamination (ie rain) and evaporation
- Nutrients – very sensitive to phosphates (soap residue on hands)
- Chl – volumetric, no bubbles
- HPLC – volumetric, filled completely; volume varies with chlorophyll concentration
- Phyto – “Pooh” sample; formalin preserved, no rinses; usually by the CTD operator
- Prodo – Primary productivity C14 uptake experiment, done at the noon station; samples drawn by the prodo person
- All sample containers (except the Phyto-Pooh sample) require 3 rinses.
- Chl filtration – ~14 chlorophyll samples are taken to the Chl van and filtered asap (usually during the Bongo net deployment)
- HPLC filtration – usually done along with chl filtration but may take much longer to complete because of larger volumes. Do not let the filters dry out.
- Net deployment & washdown – launch, recover and washdown Pairovet, Manta, and Bongo nets. Refer to the Fisheries Net Handbook for information on net types.
- Zooplankton sample “pickling” – formalin-preserve net cod-ends. Let the Chief Scientist know if you are sensitive to formalin or any other chemical.
- Secchi disc – deploy, determine secchi depth, recover
- Chl sample analysis – after 24+ hours of extraction, the chlorophylls can be measured on the fluorometer during transits between stations.
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